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We do not know what effect choosing a god for human character has on it. We know other two questions effect personal story.
We do not know what effect choosing a sprit has on norn character.
We do not know what effect choosing asura's mentor has on asura character.
We do not know if charr choice for sparring partner leads to different story lines or just effects which NPC appears in other story lines.
We do not know if sylvari's choice for time of day born leads to different story lines or just effects which NPC appears in other story lines.
We don't know what the evil faction for human is. Arenanet told us about norn, charr, asura and sylvari factions but not human. Probably because it would be a major spoiler.
We don't know how Cadeurn (a secondborn sylvari), founder of the Nightmare Court lost its leadership to Faolin (a firstborn).
We don't know the name of the Deep Sea Dragon, except that it is NOT Bubbles!
We don't know how Zaihtan will be defeated (I hope we dont just put the dragon back to sleep).
We don't know if /dance emote will be in game at launch. Or what dances will be like.
W don't know when the first expanion will be added or what it will add. Lots of fans hope tengu as playable race.
We don't know what state humanity is in in Cantha.
We don't know what state humanity is in in Elona.
We do not know what recommended system configuration is, only minimum, and even that is subject to change after optimization.
]We do not know the launch time (only date). - now known (this site needs to add striking out text)
We do not know what time servers will be live for the "three days" of early access for those who pre-purchased.